DURATION EFFECT FROM HUMAN CHORIONIC GONADOTROPHIN (hCG) INJECTION TO OOCYTE RETRIEVAL ON INTRACYTOPLASMIC SPERM INJECTION (ICSI) PERFORMANCE IN MICE
Article Sidebar
Main Article Content
Abstract
This study was conducted to compare the effect of duration from human chorionic gonadotrophin (hCG) injection to oocyte retrieval (13-15 h or 16-18 h) on intracytoplasmic sperm injection (ICSI) performance in mice. The mice were superovulated with 5 IU of pregnant mare’s serum gonadotrophin (PMSG) and subsequently 5 IU of hCG 48 h later via intraperitoneal (i.p.) injection. The oocytes were retrieved 13-15 h or 16-18 h after hCG administration. The number of ovulated oocytes showing the first polar body (PB1; metaphase II indicating matured oocytes) were higher at 13- 15 h compared to 16-18 h post-hCG injection (P < 0.05). Only oocytes with the PB1 were used for ICSI. All cleavage rates following ICSI were shown to be higher in oocytes retrieved at 13-15 h post-hCG compared to 16-18 h post-hCG injection (P < 0.05). In conclusion, it is suggested that oocytes can be retrieved at 13-15 h post-hCG injection and longer duration may be detrimental for ICSI performance in mice. ABSTRAK Kajian ini telah dijalankan untuk membandingkan kesan tempoh suntikan dari human chorionic gonadotrophin (pos-hCG) ke perolehan oosit (13-15 jam atau 16-18 jam) ke atas prestasi suntikan sperma intrasitoplasma (ICSI) dalam mencit. Mencit telah disuperovulasi dengan 5 IU pregnant mare’s serum gonadotrophin (PMSG) dan kemudian dengan 5 IU hCG jarak 48 jam secara suntikan intraperitoneum (i.p.). Oosit dipungut 13-15 jam atau 16-18 jam selepas sunitkan hCG. Bilangan oosit terovulasi diperolehi yang menunjukkan jasad kutub pertama (PB; metafasa II menunjukkan oosit matang) adalah lebih tinggi pada tempoh 13-15 jam berbanding dengan 16-18 jam suntikan pos-hCG (P<0.05). Hanya oosit dengan PB1 digunakan untuk ICSI. Semua kadar pembelahan selepas ICSI telah ditunjukkan lebih tinggi pada tempoh 13-15 jam berbanding dengan suntikan 16-18 jam pos-hCG (P<0.05). Kesimpulannnya, adalah dicadangkan bahawa oosit boleh dipungut pada tempoh suntikan 13-15 jam pos-hCG dan tempoh yang lebih panjang mungkin memnjejaskan prestasi ICSI dalam mencit. (Keywords: Post-hCG, Oocyte post-maturity, Intracytoplsmic sperm injection, Mice)
Downloads
Article Details
Transfer of Copyrights
- In the event of publication of the manuscript entitled [INSERT MANUSCRIPT TITLE AND REF NO.] in the Malaysian Journal of Science, I hereby transfer copyrights of the manuscript title, abstract and contents to the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) for the full legal term of copyright and any renewals thereof throughout the world in any format, and any media for communication.
Conditions of Publication
- I hereby state that this manuscript to be published is an original work, unpublished in any form prior and I have obtained the necessary permission for the reproduction (or am the owner) of any images, illustrations, tables, charts, figures, maps, photographs and other visual materials of whom the copyrights is owned by a third party.
- This manuscript contains no statements that are contradictory to the relevant local and international laws or that infringes on the rights of others.
- I agree to indemnify the Malaysian Journal of Science and the Faculty of Science, University of Malaya (as the publisher) in the event of any claims that arise in regards to the above conditions and assume full liability on the published manuscript.
Reviewer’s Responsibilities
- Reviewers must treat the manuscripts received for reviewing process as confidential. It must not be shown or discussed with others without the authorization from the editor of MJS.
- Reviewers assigned must not have conflicts of interest with respect to the original work, the authors of the article or the research funding.
- Reviewers should judge or evaluate the manuscripts objective as possible. The feedback from the reviewers should be express clearly with supporting arguments.
- If the assigned reviewer considers themselves not able to complete the review of the manuscript, they must communicate with the editor, so that the manuscript could be sent to another suitable reviewer.
Copyright: Rights of the Author(s)
- Effective 2007, it will become the policy of the Malaysian Journal of Science (published by the Faculty of Science, University of Malaya) to obtain copyrights of all manuscripts published. This is to facilitate:
(a) Protection against copyright infringement of the manuscript through copyright breaches or piracy.
(b) Timely handling of reproduction requests from authorized third parties that are addressed directly to the Faculty of Science, University of Malaya. - As the author, you may publish the fore-mentioned manuscript, whole or any part thereof, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
You may produce copies of your manuscript, whole or any part thereof, for teaching purposes or to be provided, on individual basis, to fellow researchers. - You may include the fore-mentioned manuscript, whole or any part thereof, electronically on a secure network at your affiliated institution, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- You may include the fore-mentioned manuscript, whole or any part thereof, on the World Wide Web, provided acknowledgement regarding copyright notice and reference to first publication in the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers) are given.
- In the event that your manuscript, whole or any part thereof, has been requested to be reproduced, for any purpose or in any form approved by the Malaysian Journal of Science and Faculty of Science, University of Malaya (as the publishers), you will be informed. It is requested that any changes to your contact details (especially e-mail addresses) are made known.
Copyright: Role and responsibility of the Author(s)
- In the event of the manuscript to be published in the Malaysian Journal of Science contains materials copyrighted to others prior, it is the responsibility of current author(s) to obtain written permission from the copyright owner or owners.
- This written permission should be submitted with the proof-copy of the manuscript to be published in the Malaysian Journal of Science